Spectroscopy Ii- Hplc & Raman

UNIVERSITI TUNKU ABDUL RAHMAN Faculty Course Year/ Semester Session : : : : Faculty of scholarship Bac. of Science (Hons) Chemistry Year 3 Semester 1 201105 or May 2011 Unit Code Unit Title reviewer Assignment : : : : UDEC3254 Spectroscopy and Chromatography II Dr. Lim Chan Kiang 2 suffice completely questions 1. A change of steroid hormones consisting of cortisone, cortexone and corticosterone was separated utilize a HPLC silica column eluted with a binary mixture of dimethyl ketone and hexane (20:80). (i) What is the ordination of elution while for the above compounds from the HPLC column? (3 marks) (ii) What is the chromatographic chemical mechanism involved? get out how the mechanism effects separation to the threesome compounds. (8 marks) (iii) With explanation, describe what would happen to the peaks in the chromatogram when the fisticuffs silica with a larger pore size and small surface stadium is used in the above separation. (6 marks) 2. A mixture of Br-, Cl- and PO43- ions was subjected to an non-ionic detergent column packed with polym-N(CH3)3+OH-, eluted with 9mM sodium carbonate. (i) define the above analyte ions in order of increasing retention time in the anionic detergent column.

(3 marks) (ii) Describe how the analyte ions were separated in the anionic column harmonise to order of retention time answered in part (2)(i). (8 marks) 3. An stranger compound is irradiated with an excitation photon. An anti-Stokes place out is observed at 439.3 nm corresponding to crusade of 2270 cm-1. i) What is the excitation photon used in the barb above? (2 marks ) ii) stripping the wavenumber and wavel! ength of the Stokes line and anti-Stokes line with shifts of 3270 cm-1 and 1170 cm-1, respectively. (6 marks) iii) A Stokes line supposed to be found in the region 490 nm to 500 nm in the Raman spectrum was swamped by fluorescent band. Describe how to solve the hassle so that the Raman shift for the Stokes line usher out be determined. (4 marks) If you want to get a full essay, order it on our website: BestEssayCheap.com

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