Microbiology

JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1980, p. 205-208 0095-1137/80/03-0205/04$02.00/0 Vol. 11, No. 3 Comparison of Selective inventory and Plating Methods for Isolation of streptococci mutans from Root Surface alveolar consonant Plaques R. P. ELLEN,`* E. D. FILLERY, AND D. W. BANTING2 Departments of Clinical and biologic Sciences, University of Toronto, cogency of Dentistry, Toronto, Canada, and Department of Community and paediatric Dentistry, University of Western Ontario, Faculty of Dentistry, London, Ontario, Canada2 Approximately 150 sound calm step ship in 44 subjects prone to root come forth infernal region were sampled longitudinally to determine the microbial flora associated with root dental cavity initiation. During the first 16 months of the study, a comparison of Streptococcus mutans retrieval was made by using three bacteriological media: mitis-salivarius agar (MSA), mitis-salivarius-bacitracin-sucrose agar (MSB), and a partiall y selective mannitol-containing broth. Total strep and S. mutans populations were found to be much lower than in former reports. MSB was more selective; S. mutans was detected in many an(prenominal) samples when its numbers were too low for isolation on MSA. However, recovery of S.

mutans was greater on MSA than on MSB for most samples subjugate colonies on both media. Mannitolcontaining broth used as an enrichment military force yielded the highest frequency of S. mutans isolation among the three media. Considering its recognized cariogenicity in science lab animals and its repeated association with coron al dental caries in humans, the frequent iso! lation of Streptococcus mutans from established root lesions whitethorn lead to its implication in the etiology of root surface caries as well. Microbiological data relating to the human root caries problem have been limited to studies of extracted teeth (6, 9), pooled plaques from populations known to do root caries (5), and single plaques overlying root lesions of unknown age (12). In the latter...If you want to get a full(a) essay, prescribe it on our website: BestEssayCheap.com

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